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We hypothesized that the use of “template-switching” to append a second PCR handle during reverse transcription might limit the recovery of unique transcripts and genes from individual cells in some massively parallel scRNA-seq methods such as Seq-Well and Drop-Seq (Gierahn et al. slot 389 login , 2017; Macosko et al. tag heuer formula 1 indy 500 , 2015)

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